pIMAGO

pIMAGO for universal, non-antibody detection of protein phosphorylation

Tymora Analytical offers pIMAGO — a novel, non-antibody, highly effective, and safe product for unbiased universal detection of any phosphoprotein.

pIMAGO is based on water-soluble, globular nanopolymers (i.e., dendrimers) that are multi-functionalized with reactive groups (e.g., Ti(IV) ions) for the highly specific recognition of phosphate groups, and with ‘reporting’ groups (e.g., biotin, fluorophore) that facilitate their detection. Multiple functionalized groups per dendrimer ensure very strong binding to the phosphoproteins (resulting in high specificity) and significant signal amplification from many “reporting” groups (resulting in high sensitivity).

pIMAGO enables universal non-antibody phosphoprotein detection in a number of formats, including:

On-membrane detection

from

$140

Formats include: Western Blot, Dot-blot format

All kits include pIMAGO-biotin reagent, streptavidin-based detection reagent, blocking buffer, pIMAGO buffer, washing buffer, and a control phosphoprotein. ECL substrate is not included.

Supplementary products are also available for purchase.

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On-microplate detection

from

$145

Formats include: ELISA, Microplate

All kits include pIMAGO-biotin reagent, streptavidin-based detection reagent, blocking buffer, pIMAGO buffer, high-binding adsorption plates and a control phosphoproteins. HRP-based kits also include colorimetric substrate and stop solution.

Supplementary products are also available for purchase.

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Microarray detection

We are currently in the process of developing a pIMAGO system for microarray-based detection (peptide/protein arrays, kinase substrate arrays, antibody array). Please contact us for more information or for collaborative opportunities.

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pIMAGO Advantages:

Good quantitative capabilities and linearity
Ability to detect in vivo phosphorylated proteins after immunoprecipitation
Sensitive and specific universal detection of phosphoproteins
Multiplexed detection capabilities of pIMAGO with total protein antibodies
Effectiveness for in vitro kinase and phosphatase assays
Efficacy for kinase activity profiling and inhibitor screening
Capable of selective binding to a phosphorylated residue independent of amino acid sequence, unlike antibodies