pIMAGO for universal, non-antibody detection of protein phosphorylation
Tymora Analytical offers pIMAGO — a novel, non-antibody, highly effective, and safe product for unbiased universal detection of any phosphoprotein.
pIMAGO is based on water-soluble, globular nanopolymers (i.e., dendrimers) that are multi-functionalized with reactive groups (e.g., Ti(IV) ions) for the highly specific recognition of phosphate groups, and with ‘reporting’ groups (e.g., biotin, fluorophore) that facilitate their detection. Multiple functionalized groups per dendrimer ensure very strong binding to the phosphoproteins (resulting in high specificity) and significant signal amplification from many “reporting” groups (resulting in high sensitivity).
pIMAGO enables universal non-antibody phosphoprotein detection in a number of formats, including:
On-membrane detection
from
$140
On-microplate detection
from
$145
Formats include: ELISA, Microplate
All kits include pIMAGO-biotin reagent, streptavidin-based detection reagent, blocking buffer, pIMAGO buffer, high-binding adsorption plates and a control phosphoproteins. HRP-based kits also include colorimetric substrate and stop solution.
Supplementary products are also available for purchase.
pIMAGO Advantages:
pIMAGO Advantages:
Good quantitative capabilities and linearity
Sensitive and specific universal detection of phosphoproteins
Effectiveness for in vitro kinase and phosphatase assays
Capable of selective binding to a phosphorylated residue independent of amino acid sequence, unlike antibodies
Ability to detect in vivo phosphorylated proteins after immunoprecipitation
Multiplexed detection capabilities of pIMAGO with total protein antibodies
Efficacy for kinase activity profiling and inhibitor screening